Zeocin
Selection Reagent
● 產品規(guī)格
Zeocin粉末型
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名稱
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貨號
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規(guī)格
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貯存溫度
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運輸溫度
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Zeocin (干粉)
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RT250-F
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100 mg
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-20℃
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常溫
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Zeocin 溶解液
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1 ml
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4℃
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常溫
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● 產品說明
Zeocin?
Reagent is a member of the bleomycin antibiotic family. Resistance is conferred
by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing
the protein, Zeocin? is prevented from binding and cleaving the cellular DNA.
Zeocin? is effective on most aerobic cells and can be used for selection in
mammalian and insect cell lines, yeast, and bacteria. The amount of Zeocin?
required for selection will vary between 50-2000 μg/ml (typically 300μg /ml)
depending on the cell type.
Zeocin可選擇Sh
ble基因表達的細胞。與現在使用的其他動物細胞標記物無交叉抗性。因此這種抗生素可用來分離對其他篩選劑（如：慶大霉素，潮霉素）有抗性的克隆。Zeocin是一種屬于爭光霉素家族的糖蛋白抗生素，在體內能作用于大多數細菌（包括E. coli）、真菌（如：酵母菌）、植物細胞、動物細胞。
● 液體Zeocin可用于細胞培養(yǎng)。
●
用于篩選轉化株的Zeocin濃度根據pH值和鹽濃度改變。pH越高，鹽濃度越低，Zeocin活性越大。
●
普通大腸桿菌菌株篩選可在25
μg/ml zeocin濃度的低鹽LB瓊脂培養(yǎng)基（yeast
extract 5 g/L, Tryptone 10 g/L, NaCl 5 g/L, Agar 15 g/L,pH 7.5）中進行，pH調至7.5
。
● 動物細胞篩選：根據不同細胞系從100 到
750 μg/ml。
● 植物細胞篩選：根據不同的細胞種類和培養(yǎng)基從50 到
250 μg/ml 。
●
酵母篩選：取決于種屬和培養(yǎng)基，S. cerevisiae Sh ble轉化株在50
μg/ml 濃度的YPD培養(yǎng)基中篩選，pH調至7.0。
● 粉末型配制方法：
取1 ml Zeocin溶解液加到Zeocin干粉中，徹底溶解，用0.22
μm 一次性濾器抽濾除菌，即配成1
ml（100mg/ml）的Zeocin溶液，-20℃保存。
● 技術參數：
Empirical formula: C55H85O21N20S2Cu·HCl
Molecular weight: 1525
CAS：11006-33-0
Zeocin
使用該產品發(fā)表部分文章列表：
1. [2022 IF=11.4] Random mutagenesis
and transcriptomics-guided rational engineering in Zygosaccharomyces rouxii for
elevating D-arabitol biosynthesis.
Author: Guoyan Zhang, Hossain M Zabed, Yufei Zhang, Jia Li, Junhua Yun,
Xianghui Qi
Journal: Bioresource Technology 400 (2024) 130685
Institution： School
of Life Sciences, Guangzhou University
Paper link：https://doi.org/10.1016/j.biortech.2024.130685